HPLC测定地耳草中槲皮苷的含量

Determination of Quercitrin in Hypericum japonicum Thunb.by HPLC

目的测定地耳草中槲皮苷的含量。方法采用高效液相色谱法,AgilentC18柱(250mm×4.6mm,5μm),流动相为乙腈-0.05mol·L-1磷酸二氢钾溶液(19∶81),检测波长为256nm,流速为1.0mL·min-1。结果槲皮苷进样量在0.1204～2.408μg内呈良好的线性关系,平均回收率为96.0%,RSD=1.3%(n=6)。结论该方法快速简便,稳定可靠,专属性强。

OBJECTIVE To determine the content of quercitrin in Hypericum japonicum Thunb. by HPLC. METHODS An Agilent C18 column（250 mm×4.6 mm, 5 μm） was used. The mobile was acetonitrile-0.05 mol·L^-1 potassium dihydrogen phosphate（19 ： 81）, and detected at 256 nm with flow rate of 1.0 mL·min^-1. RESULTS A good linearity was observed for quercitrin in the range of 0.120 4-2.408 μg. The average recovery of quercitrin was 96.0% with RSD of 1.3%（n=6）. CONCLUSION The method is convenient, stable, reliable and special for the dermination of quercitrin in Hypericum japonicum Thunb..