探头式超声提取三七有效成分的工艺研究

Ultrasound-assisted extraction of the active components of Panax notoginseng with ultrasonic horn

目的采用单频探头式超声（25kHz）技术对三七有效成分进行提取。方法选择乙醇体积分数、物料粒径、提取固液比、提取温度、提取时间5个因素进行正交实验。结果影响三七药材中人参皂苷Rg1的提取率的因素依次为：乙醇体积分数〉物料粒径〉固液比〉提取时间〉提取温度，优化工艺参数为：乙醇体积分数95％，物料粒径80～100目，固液比1：15，提取时间40min，提取温度40℃。在此条件下，人参皂苷Rg1的提取率是69．38％。结论本研究将为三七工业化生产工艺参数的选择提供实验依据。

Objective To extract the active components of Partax notoginseng by single-frequency ultrasound wave. Methods The concentration of alcohol, size of material, ratio of solid to liquor, temperature and time of extraction were optimized by orthogonal design. Results The factors influencing extraction rate of ginsenoside Rg1 in Panax notoginseng included ethanol concentration, particle size, solid/liquor, temperature and extraction time. Their optimal parameters were： 95% of ethanol concentration, particle size 80 -100 mu; solid/liquid ratio 1：15 g/mL,ultrasound time 40 rain,ultrasound extraction temperature 40 ℃. Under this optimal condition,the yield of ginsenoside Rg1 was 69.38%. Conclusion This method provided useful parameters for the extraction of Panax notoginseng.