蒲金丹醒脑液对急性实验性脑缺血家兔脑保护作用的实验研究

EXPERIMENTAL STUDY ON THE PROTECTIVE FUNCTION FOR BRAIN OF PUJINDANXINGNAO SOLUTION ON RABBIT WITH ACUTE EXPERIMENTAL CEREBRAL ISCHEMIA

为了探讨中药蒲金丹醒脑液对急性脑血管疾病的治疗作用,采用结扎兔双侧颈总动脉造成急性脑缺血模型,以安宫牛黄丸为阳性对照组,观察其对脑组织含水量,脑脊液乳酸脱氢酶,脑组织丙二醛、超氧化物歧化酶以及钙离子含量的影响。实验结果表明,脑缺血形成后,脑组织水、丙二醛、钙离子含量明显增加,超氧化物歧化酶含量明显降低,脑脊液乳酸脱氨酶含量亦明显增加。而蒲金丹醒脑液和安宫牛黄丸均能不同程度地减轻脑组织损伤,具有脑保护作用,改善以上各指标的变化,其作用优于安宫牛黄丸组。由此证实蒲金丹醒脑液对急性脑血管病有一定疗效。

In order to approach the mechanism of pujindanxingnao solution actting on acute cerebral ischemia, ex-]>erimental observation on the solution effect on animal model with acute cerebral ischemia was done, the an-gongnluhuang pill was used as positive control medicine. The water content of brain tissue, LDH in eerr-brospinal fluid, MDA, SOD, and Ca' + contents in brain tissue were the observed indexes. The results showed that water, MDA and Ca + contents in braintissue were significantly increasing. SOD was decreasing, LDH in cerebrospinal fluid was obviously increasing as the formation of cerebral ischemia. ftyindanxingnao solution and angongniuhuang pill both improved the change of above indexes and abated the damage of brain tissue at different degree. the protective function of solution was better than that of positive pill. All these suggested that Pujindanxingnao solution was effective formula on acute cerebrovascular disease.