Has-mir-129影响人肾细胞癌原位移植裸鼠肺转移模型的实验研究

Experimental Study of Has-mir-129 Impacted Orthotopic Mouse Model of Human Renal Cell Carcinoma with Pulmonary Metastasis

目的:建立人肾细胞癌原位移植裸鼠肺转移动物模型,研究Has mir-129对肾脏移植瘤和肺部转移瘤的影响。方法:构建Has-mir-129慢病毒载体,以人肾癌细胞株SN12-PM6建立的动物模型作为对照组,以Has-mir-129慢病毒载体感染肾癌细胞建立的动物模型作为实验组;7周后观察裸鼠肾脏肿瘤及肺部转移瘤的生长情况,并分别计算两组的肾脏成瘤率、肺部转移率及肿瘤的重量;所有标本均采用10%甲醛溶液固定,常规石蜡包埋、切片、HE染色后分析结果。结果:对照组肾脏成瘤率为100%(8/8),肺部转移率为100%(8/8),肿瘤重量平均为(0.712 8±0.147 4)g;实验组肾脏成瘤率为100%(8/8),肺部转移率为62.5%(5/8),肿瘤重量平均为(0.481 5±0.111 2)g;肾脏成瘤率两者之间差异无统计学意义,肿瘤重量和肺部转移率差异有统计学意义(P<0.05)。结论:成功建立了Has-mir-129慢病毒载体及人肾细胞癌原位移植裸鼠肺转移动物模型,发现Has-mir-129能抑制人肾细胞癌原位移植裸鼠肺转移模型移植瘤的生长及肺部转移瘤的形成。

Objective：To construct orthotopic mouse model of human renal cell carcinoma with pulmonary metastasis and to study the impact of miRNA 129 on renal transplantation tumor and lung metastases. Methods： We constructed the lentiviral vector of has-mir-129 and set up the animal model of human renal cancer cell line SN12- PM6 being infected with the lentiviral vector as the experiment group. And we set up the animal model of human renal cancer cell line SN12-PM6 as the control group. After 7 weeks tumorigenicity and metastasis were evaluated subsequently. In the two groups, the percentage of tumorigenicity and metastasis was calculated; at the same time, the weight of tumor was also measured. All tumor tissues were fixed in 10% formalin and embedded in paraffin routinely. Paraffin-embedded tissues were sectioned and stained with hematoxylin and eosin （HE） for routine histological examination. Results：In the control group, the percentage of tumorigenicity was 100 % （8/8）, the percentage of metastasis was 100%（8/8） and the average tumor weight was（0.712 8±0. 147 4）g. But in the experiment group, the percentage of tumorigenicity was 100%（8/8）, the percentage of metastasis was 62.5% （5/8） and the average tumor weight was（0.481 5 ± 0. 111 2）g. Statistics show that the percentage of tumorigenicity was not significant different between the two groups, but the average tumor weight and the percentage of metastasis was significantly different between the two groups（P〈0.05）. Conclusions：We have successfully constructed has-mir-129 Impacted orthotopic mouse model of human renal cell carcinoma with pulmonary metastasis. Has-mir-129 can inhibit tumor growth and puhnonary metastasis of orthotopic mouse model of human renal cell carcinoma with pulmonary metastasis.