IL-6、IL-6 R及VEGF-C在前列腺癌中的表达及其临床意义

The Expression and Clinical Significance of IL-6,IL-6R and VEGF-C in Prostate Cancer

目的:研究前列腺癌组织中白细胞介素6(IL-6)及其受体(IL-6R)和淋巴管内皮生长因子-C(VEGF-C)的表达水平及其相关性,探讨三者与前列腺癌生物学行为之间的关系。方法:①采用酶联免疫吸附反应(enzyme-Linked immunosorbent assay,ELISA)检测26例前列腺癌及其相应癌旁组织中IL-6、IL 6R的表达水平;②运用免疫组织化学SP法检测前列腺癌及其相应癌旁组织中VEGF-C的表达情况。结果:①前列腺癌组织中IL-6、IL-6R的表达水平较相应癌旁组织显著提高(P<0.05),且二者呈明显正相关;②前列腺癌中VEGF-C的表达明显强于相应癌旁组织(P<0.05);③IL-6、IL-6R和VEGF-C在前列腺癌中的表达水平与肿瘤的临床分期、分化程度、有无淋巴结转移等生物学行为显著相关。结论:①IL 6与IL6R协同作用,可能在前列腺癌的发生、发展中起重要作用;②VEGF-C与前列腺癌淋巴管浸润和转移有显著相关性,检测前列腺癌VEGF-C的表达能预示盆腔淋巴结是否转移。

Objective：To study the expression and correlation of interleukin 6 （IL-6）, IL-6 receptor （IL-6R） and vascular endothelial growth factor C （VEGF-C） in the prostate cancer, and to investigate the relationship between IL-6IL-6R , VEGF-C and the biological behavior of prostate cancer. Methods：① Enzyme-linked immunosorbent assay （ELISA） was used for detection of the expression level of IL-6 and IL-6R in 26 cases prostate cancer and adjacent tissues. ② Immunohistochemistry method was used to detect the expression of VEGF-C protein in prostate cancer and adjacent tissue. Results：①The expression level of IL-6 and IL-6R in cancer tissues were significantly higher than those in their adjacent tissues with a positive correlation between IL-6 and IL-6R levels （P〈 0. 05）. ②The expression of VEGF-C in prostate cancer tissue were significantly higher than those in adjacent tissue （P〈0.05）. ③The expressions of IL-6, IL-6R and VEGF-C in neoplastic tissues of patients were positively related to the clinical stage, tumor grade and the lymph node metastasis of cancer cells. Conclusions：①The synergy of IL-6 and IL-6R may play an important role in prostate cancer progression. ②VEGF-C expression may be significantly associated with the lymphatic invasion and metastases in prostate cancer, Examination of VEGF-C expression may be beneficial in the prediction of pelvic lymph node metastasis of prostate cancer.