洗必泰影响肺炎克雷伯菌形成生物被膜的体外研究

In vitro effect of chlorhexidine on biofilm formation of Klebsiella pneumoniae

目的研究肺炎克雷伯菌(KP)生物被膜对洗必泰(CHX)的敏感性。方法在聚苯乙烯棒上培养KP形成生物被膜。银染法鉴定生物被膜,用不同浓度的CHX治疗。用扫描电镜观察生物被膜变化,紫外分光光度计测定聚苯乙烯棒表面生物量的变化。结果CHX对KP形成的生物被膜有明显分解作用,以2mg/mlCHX效果较好。结论CHX可被用作抗生物被膜药剂。

Objective To study the sensitivity of Klebsiella pneumoniae （KP） biofilm to chlorhexidine. Methods KP was cultured to form adherent bioflm on the surface of polystyrene rods. The biofilm was identified by silver nitrate dyeing method and treated with different concentrations of chlorhexidine. The variation of biofilm was examined by scanning electron microscope. He）dos UV was used to measure the biomass. Results Cblorhexidine had an obvious decomposition effect on the KP biofilm, for which 2 mg/ml of chlorhexidine was better. Conclusion Chlorhexidine is an useful kind of anti-biofilm agents.