扶桑花醚不溶物HR-2对体外培养的人绒毛膜组织及小鼠离体子宫活动力的影响

Effect of Petroleum Ether Insoluble Fraction of Hibiscus rosa sinensis flowers(HR-2) on Human Trophoblast cells and Uterus Activity of Mice in vitro

不同浓度的扶桑花醚不溶物ＨＲ－２对体外培养的人绒毛膜组织的形态、ＨＣＧ和孕酮分泌的影响．观察了ＨＲ－２对小鼠离体子宫的刺激作用及不同阻断剂对子宫活动力的影响．结果表明：１）ＨＲ－２可以明显抑制人绒毛膜组织细胞的正常生长．２）培养液中的孕酮含量随ＨＲ－２的浓度增加呈梯度下降（Ｐ＜０．０１）；而ＨＣＧ仅在高浓度时有明显降低．３）ＨＲ－２对离体的小鼠子宫有直接的兴奋作用（Ｐ＜０．０１），这种兴奋作用不被消炎痛、阿托品和心得安阻断，但可被异丙嗪完全阻断．

Present communication deals with the effect of petroleum ether insoluble fraction of Hibiscus rosa sinensis.flowers(HR-2) on human trophoblast cells morphology, HCG and progesterone secretion, and investigates the action of HR-2 on uterine smooth muscles of mice, as well as the effect of different blocking drugs on HR-2. The results indicate that:(1) HR-2 has a significant inhibition on the morphology of human trophoblast cells,(2) the concentration of progesterone in cultural medium decrease with the increase of HR-2 doses (P<0.01),but HCG reduces only when the dose of HR-2 is 100 μg/ml and 200 μg/ml.(3)HR-2 has a direct stimulation on uterus of mice in vitro (P<0.01), and this stimulation is related to H1 receptor of uterine smooth muscles.