摘要
目的构建基于人乳头状瘤病毒(HPV)16型的伪病毒载体,并检测其对肝癌细胞系HepG2细胞的杀伤活性。方法利用昆虫杆状病毒表达系统表达病毒蛋白,在体外将病毒蛋白包装白喉毒(DT)A链表达型质粒,形成伪病毒。透射电镜观察病毒样颗粒(VLP)的结构,并转染肝癌细胞系HepG2,乳酸脱氢酶释放法检测对肝癌细胞系HepG2的杀伤能力。结果透射电镜观察显示病毒蛋白可自我组装成VLP,在转染肝癌细胞系HepG2后,乳酸脱氢酶释放法成功检测到伪病毒对肝癌细胞系HepG2的杀伤作用。结论基于HPV16的新型伪病毒载体能成功转染肝癌细胞系HepG2,并产生杀伤活性,为肝癌的基因治疗提供了一种可选择的方法。
Objective To construct human papilloma virus type 16 (HPV16) vector and examine its killing activity on HepG2 cells. Methods Virus protein was expressed by Bac-to Bac expression system, DT Aexpression plasmid together with virus protein were formed in vitro. The construction was identified by transmission electron microscope, the transfection and killing activity of the pseudovirus were detected by LDH release assay. Results The pseudovirus could selfassemble into VLP,LDH release assay could detect the killing activity successfully. Conclusion Pseudovirus vector of human papilloma virus type 16 could transfect and kill HepG2 cells, which might provide a new method for liver cancer therap.
出处
《肝脏》
2009年第3期204-205,共2页
Chinese Hepatology