海南地区宫颈癌的HPV基因型检测及其临床意义

Detection of HPV Genotypes of Cervical Cancer and the Clinical Significant in Hainan

目的:通过对宫颈癌组织的HPV分型检测,了解本地区宫颈癌HPV感染率及其亚型的分布情况,并分析HPV感染与宫颈癌临床生物学行为之间的关系。方法:收集200例宫颈癌患者病灶组织标本,采用HPV分型基因芯片检测系统,同时对23种HPV亚型(18种高危型和5种低危型)进行检测。结果:①HPV阳性率94.00%,共检出HPV16(69.68%)、HPV18(16.49%)、HPV33(1.06%)、HPV45(1.60%)、HPV58(1.06%)、HPV73(0.53%)6种高危型亚型。其中HPV16+18(7.45%)、HPV16+45(1.06%)、HPV18+33(1.06%);②鳞状细胞癌的HPV检出率明显高于腺癌(P<0.05);③HPV阳性率与宫颈癌患者的年龄、临床分期、病理组织学分级、盆腔淋巴结转移、治疗后的复发差异均无统计学意义(P>0.05)。结论:本组宫颈癌的HPV感染率很高,且均为高危型,其中以16型和18型占绝对优势;HPV亚型比较局限,仅有6种;HPV多重感染并不多见;高危HPV的存在与鳞状上皮恶变关系密切。

Objective：To understand HPV prevalence and HPV genotypes distribution of cervical cancer in Hainan area though the inspection of HPV subtypes, and to analyze the relation of HPV and clinical biological behavior in cervical cancer. Methods：The tissue samples of 200 cervical cancers were collected. HPV genotyping DNA chip assay was used to detect 23 HPV genotypes simultaneously, including 18 high-risk subtypes and 5 low-risk subtypes. Results：The positive rate of HPV infection was 94.00% and all ware high-risk subtypes, including 16, 18, 33, 45, 58, and73 （HPV1669.68%, HPV18 16.49%, HPV16＋ 18 7.45%, HPV45 1.59%, HPV33, 58, 16＋45, 18＋33 1.06%, and HPV73 0.53% ）. HPV positive rate was higher in squamous cell carcinoma than in adenocaminorr~ （ P 〈0.005）; The positive HPV rate had no relationship with patients＇ age, clinical stage, pathological grade, the metastasis of lymph nodes, and recur in cervical cancer （P〉O.05）. Conclusions： HPV infection of cervical cancer is very high in these cases with all high-risk subtypes. HPV16, 18 are absolute superiority, but HPV subtypes are limited, only6 strains. Multiple-infection is not common： HPV infection was more closely with the malignance of squarnous cell.