摘要
采用SDS凝胶电泳方法从人脊神经前根中分离出运动神经元特有的蛋白组份190KD蛋白。将该蛋白作为抗原,免疫BALB/C小鼠,经杂交瘤技术,获得抗190KD蛋白的单克隆抗体。免疫细胞化学检测表明,190KD单抗与脊髓灰质运动神经元、前根及肌支发生阳性反应。实验结果提示,190KD蛋白分布在脊髓运动神经元胞体及其轴突和肌支纤维中。
The 190KD protein of motor nerve was isolated from the anterior roots of human spinal cord by the SDS-PAGE. The monoclonal antibody was prepared through immunizing the BALB/C mouse with 190KD protein. The results of the immunocytochemical method showed that positive products distributed in motor neurons in spinal cord, the fibers of anterior roots, and muscular branches of peripheral nerve. This study suggested that the 190KD protein exists only in the motor neurons, anterior roots and muscular branches.
出处
《南通医学院学报》
1998年第3期293-295,共3页
ACTA Academiae Medicinae Nantong
基金
国家杰出青年科学基金
江苏省教委青蓝工程资助