高压氧对老年小鼠记忆能力的研究

Study of Hyperbaric Oxygenation on Memory Ability in Aged-mice

观察常压（101．3kPa）及高压（253．3kPa）吸氧处理后老年小鼠行为及学习记忆的改善情况，并测定四个主要脑区（皮层、海马、小脑、间胞）突触体内钙离子浓度。结果表明，常压及高压吸氧可明显增强老年小鼠在新异环境中的自发活动能力，并且显著提高记忆保持力，对衰老引起的脑内高钙现象具有明显的抑制效应，其中253．3kPa高压氧降钙效果更明显。脑内高钙与记忆衰退密切相关，提示高压氧可用于治疗脑老化引起的学习记忆障碍。

The spontaneous behavior and the learning memory retention of aged-mice were assessed using open field and one-trial passive avoidance task after atmospheric(101.3 kPa) oxy-gen or hyperbaric(253.3 kPa) oxygen treatment(HBOT). At the same time, the synaptosomal free calcium concentration in some main brain regions (cerebral cortex, hippocampus, cerehellum and diencephalon) as measured by fluorescent probe [Ca2+], indicator Fura-2/AM and an AR-CM-MIC cation measurement system. The results showed that the spontaneous behavior and explo-rative response in the open field were significantly increased and the memory retention was re-markably elevated. The high synaptosomal free Ca2+ concentration caused hy decrepit was de-creased after atmospheric oxygen or HBOT, and there was a hetter inhibiting action of HBOT on high level of calcium in hrain. The memory declination was closely related with hypercalcemic phe-nomenon in brain. It is indicated that the senile memory impediment can be treated by HBOT.