套式聚合酶链反应检测自然流产组织中人细小病毒B19

The study on detection of human parvovirus B19 DNA in spontaneous abortion tissues

目的进一步了解我国胎儿人细小病毒Ｂ１９的感染状况及其与自然流产之间的关系。方法用所建立的套式聚合酶链反应（ＰＣＲ）技术对５０例新鲜的和６６例石腊包埋的自然流产组织及２５例同期健康孕妇人工流产组织进行人细小病毒Ｂ１９ＤＮＡ检测。结果显示病例组有３４例阳性，阳性率为２９．３％，而正常对照组有１例阳性，阳性率为４％。经统计学检验有显著性差异。结论可能人细小病毒Ｂ１９感染与自然流产有一定关联。

116 spontaneous abortion tissues were detected for human parvovirus B19 (HPV B19)DNA by nested polymerase chain reaction. Of the abortion tissues detected,66 were paraffin embedded and 50 were fresh abortion tissues,At the same time,25 abortion tissues from healthy artificial abortion women were collected as control. The results showed that 27.3%(34/116) of 116 spontaneous abortion tissues were positive for HPV B19 DNA,and only 4%(1/25)were positive in the control group,the discrepancy was significant χ2=5.769(P<0.05).It suggests that there is a relationship between spontaneous abortion and HPV B19 infection.HPV B19 infection may be an important factor in spontaneous abortion.