Epstein-Barr病毒壳抗原gp125的纯化和应用

The purification and application of Epstein-Barr virus gp125

目的研制ｅｐｓｔｅｉｎ－Ｂａｒ（ＥＢ）病毒诊断试剂。方法将重组痘苗病毒表达的Ｅｐｓｔｅｉｎ－Ｂａｒ病毒（ＥＢＶ）壳抗原（ＶＣＡ）主要多肽ｇｐ１２５纯化，作为诊断抗原建立了酶联免疫吸附试验（ＥＬＩＳＡ），检测了４８份鼻咽癌（ＮＰＣ）病人血清及１０份正常人血清中的ＶＣＡ／ＩｇＡ抗体。结果该方法与免疫荧光（ＩＦ）检测结果一致，但ＥＬＩＳＡ的平均几何滴度（ＧＭＴ）是ＩＦ的１２倍。结论以纯化的ＥＢ病毒壳抗原主要多肽ｇｐ１２５作为诊断抗原建立的检测方法，更适合于ＥＢＶ相关疾病的血清学诊断和血清流行病学调查。

Epstein-Barr virus (EBV)virion capsid antigen (VCA)gp125 as a diagnostic antigen was expressed in infected cells by recombinant vaccinia virus. The presence of IgA antibody to VCA of EBV was tested by ELISA in sera from nasopharyngeal carcinoma (NPC) patients and normal individuals. The ELISA consisted of three steps involving test serum,mouse monoclonal antibody to human IgA and rabbit anti-mouse IgG.The antibody titers of sera determined by this way correlated well with those by IF.The GMT by ELISA was 12 times higher than that by IF.Furthermore,efforts to develop gold-conjugated immunosorbent assay (GCISA),which is a more sensitive assay for measuring IgA antibody to VCA,has met with initial results.