摘要
目的探讨基质金属蛋白酶-2(MMP-2)特异性抑制剂1-(12-羟)18烷基硫酸钠对负透镜诱导豚鼠离焦性近视形成的影响及其作用机制。方法对新生健康有色豚鼠随机分为3组,A组(10只):右眼进行-IOD透镜诱导,左眼不作处理;B组(10只):右眼透镜诱导+抑制剂(9.0μg/ml,每3d结膜下注射),左眼不作处理;C组(10只):右眼透镜诱导+赋形剂,左眼不作处理,4周后测量屈光度,明胶酶谱法分析后部巩膜MMP-2活性,western—blot技术检测后部巩膜MMP-2的表达。结果豚鼠经4周负透镜诱导后,A组诱导眼较对侧眼增加了(5.73±0.36)D近视度;B组诱导眼较对侧眼增加了(2.58±0.33)D近视度;C组诱导眼较对侧眼增加了(6.31±0.73)D近视度;B组所获得的近视度低于A组和C组,差异有统计学意义(q检验,P〈0.05),A组和c组相比差异无统计学意义(P〉0.05)。B组抑制剂注射眼后部巩膜MMP-2活性成分低于A组和C组,差异有统计学意义(P〈0.05),酶原成分3组问比较差异无统计学意义(P〉0.05)。3组后部巩膜MMP-2蛋白水平差异无统计学意义(P〉0.05)。结论9.0μg/ml 1-(12-羟)18烷基硫酸钠,每3d结膜下注射可通过抑制后部巩膜MMP-2的活性,从而在-定程度上抑制新生豚鼠负透镜诱导离焦性近视的发展。
Objective To investigate the inhibitory effects of Sodium 1-( 12-Hydroxy)octadecanyl Sulfate, an MMP-2 inhibitor, on the development of defocus-induced myopia in guinea pigs and its mechanism. Methods Thirty newborn guinea pigs were randomly divided into 3 groups,group A: -10D lens applied on right eye;group B: -10D lens and MMP-2 inhibitor treatment in right eye (9.0μg/ml, subconjunctival injection, every 3 days interval ) ;group C: -10D lens and vehicle treatment in right eye (same volume and frequency with those of group B ) ; there was no treatment in all the left eyes in this study.After 28 days of defocus, the refractive status was recorded and the posterior scleral sample was collected for MMP-2 zymography and western-blot analysis.Results The treated eyes of group A,B and C gained (5.73±0.36)D, (2.58±0.33)D and (6.31±0.73 )D respectively,the amount of gained myopic refraction in eyes of group A was significantly less than that of eyes in group B and group C (P〈0.05) ; zymography analysis indicated that, of all the defocused animals, the activity of MMP-2 in the eyes treated by inhibitor was significantly lower than that of eyes treated by vehicle and no injected eyes, represented by the lower level of active MMP-2 (P〈0.05);no significant difference was detected in the latent MMP-2 level between three groups (/)〉0.05 ) ; the MMP-2 protein level in posterior sclera of three groups also didn't show any significant difference by western-blot analysis (p〉0.05).Conclusions 1-( 12-Hydroxy)octadecanyl Sulfate(9.0μg/ml,subconjunctival injection,every 3 days interval)inhibited the development of defocus-induced myopia in newborn guinea pigs, may through, at least in part, in-hibitory effects on the activity of MMP-2 in posterior sclera.
出处
《中国实用眼科杂志》
CSCD
北大核心
2009年第7期771-775,共5页
Chinese Journal of Practical Ophthalmology
基金
国家自然科学基金资助项目(30600692)