乙/丙型肝炎病毒双重感染患者前C区终止变异低频率

Low frequency of precore stop mutation in patients with dual infection of hepatitis B and C viruses

目的了解乙型肝炎病毒（ＨＢＶ）与丙型肝炎病毒（ＨＣＶ）双重感染患者前Ｃ区基因变异，及其可能的临床意义。方法用聚合酶链反应（ＰＣＲ）与限制片段长度多态性（ＲＦＬＰ）来分析２５例ＨＢＶＤＮＡ和ＨＣＶＲＮＡ均阳性（Ａ组）和３１例ＨＢｓＡｇ和ＨＢＶＤＮＡ阳性但抗－ＨＣＶ和ＨＣＶＲＮＡ均阴性（Ｂ组）的慢性肝病患者前Ｃ区密码２８终止变异（终２８）。结果ＨＢＶ和ＨＣＶ双重感染患者（Ａ组）血清ＨＢＶＤＮＡ第１次ＰＣＲ阳性率（１６％）明显低于单独ＨＢＶ感染组（６５％）（Ｐ＜０．００１）；前Ｃ终２８检出率（２８％）亦明显低于单独ＨＢＶ感染（６８％）（Ｐ＜０．００１）。

In order to study the mutation of hepatitis B virus(HBV) precore gene in patients with dual infections of HBV and HCV and its possible clinical significance,the precore stop codon mutation (stop 28) was analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphism(RFLP) in patients with chronic liver disease,which were 25 cases with HBV DNA and HCV RNA positive(group A)and 31 cases with HBsAg and HBV DNA positive but anti-HCV and HCV RNA negative (group B).The results showed that the serum HBV DNA positive rate by lst PCR in patients with dual infections(group A,16%)was significantly lower than that in patients with HBV infection alone (group B,65%),the stop 28 positive rate was also significantly lower (28%,versus 68%).The results suggest that the low frequency of precore stop codon mutant in patients with HBV and HCV dual infections was possibly due to low replication of HBV.