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等电聚焦电泳测定糖化血红蛋白 被引量:2

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摘要 建立超薄层平板聚丙烯酰胺凝胶等电聚焦电泳(PAGIEF)的方法测定糖化血红蛋白(HbA1c)。取正常人及糖尿病人血97例,制成血红蛋白溶液,使用瑞典LKB公司等电聚焦电泳及激光扫描设备,用0.5mm聚丙烯酰胺平板凝胶及两性载体电解质进行电泳分离,凝胶经固定、染色、脱色后,激光扫描定量。糖化血红蛋白对照组平均值x±s=5.04%±1.43%,糖尿病人11.17%±2.25%,P<0.01,血糖值与糖化血红蛋白值有显著相关性,r=0.936,回归方程Y=0.762+0.900X。该法与其他方法相比,标本用量少,分辨率高,重复性好。
出处 《临床检验杂志》 CAS CSCD 北大核心 1998年第4期209-210,共2页 Chinese Journal of Clinical Laboratory Science
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参考文献2

  • 1沈钰如,国外医学.内分泌学分册,1995年,15卷,86页
  • 2何忠效,等电聚焦,1985年,91页

同被引文献21

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