摘要
目的建立血清新喋呤的酶免疫测定方法，并确定血清新喋呤的正常范围。方法通过碳二亚胺将新喋呤分别与牛血清白蛋白、鸡白蛋白连接制成新喋呤蛋白质交联物，用新喋呤牛血清白蛋白交联物免疫Ｂａｌｂ／ｃ鼠，通过细胞融合筛选出产生抗新喋呤的单克隆抗体，并采用该单克隆抗体建立了血清新喋呤的酶联免疫吸附竞争抑制法。结果４１２例献血员（正常组）血清新喋呤为５２±２２μｇ／Ｌ（ｘ±ｓ），４９例消化道恶性肿瘤为１４８±１０５μｇ／Ｌ（ｘ±ｓ），２１例皮肌炎为１６３±１１４μｇ／Ｌ（ｘ±ｓ）。ｔ检验表明，采用酶联免疫吸附法测定，消化道恶性肿瘤组、皮肌炎组的血清新喋呤与正常组比较Ｐ值均＜０．００１。本方法检测的批内变异系数（ＣＶ）为００３６３，批间ＣＶ为００９１７。结论本法操作简便、方法敏感、正常值范围明确、重复性好。
Objectives To establish a new method for detection of neopterin in serum and to determine the normal value of neopterin in donors.Methods Proteins compared in the presence of EDC reagent, and then, monoclonal antibodies (McAb) to neopterin were screened by the technique of cell fusion. A competitive enzyme immunoassay was used by above McAb for detection of neopterin.Results The concentration of neopterin was 5.2±2.2 μg/L (±s) in 412 donors, 14.8±10.5 μg/L (±s) in 49 patients with malignant digestive carcinoma, and 16.3±11.4 μg/L (±s) in 21 patients with skin disease, respectively. Neopterin in the latter two groups was significantly increased (P<0.001). Conclusion It is a sensitive and stable method for clinical detection of neopterin.
基金
浙江省卫生厅资助
关键词
新喋呤
血清诊断
ELISA
Enzyme linked immunosorbent assay Neopterin
