海绵分离富集尿内17-酮类固醇和17-羟皮质类固醇的测定

Detection of 17-ketosteroid and 17-hydroxycorticosteroidin urine after separation and collection by foam rubber

用聚氨脂海绵对人尿内１７－酮类固醇（１７－ＫＳ）和１７－羟皮质类固醇（１７－ＯＨＣＳ）进行分离和富集。用乙醇解吸附，分别用间二硝基苯和盐酸苯肼显色，分光光度法测定。本法标准差为±０．０３２，检出限为０．０１μｇｍｌ－１，回收率为９９．０％～１０１．０％，变异系数小于６．２３％。

Using foam rubber 17-ketosteroid (17-KS) and 17-hydroxycorticosteroid (17-OHCS) were separated and collected in urine. 17-KS and 17-OHCS were eluted down from the foam rubber with alcohol, colour reaction can be respectively produced by mdinitrobenzene and phenyhydrazine hydrocholoride, then both of them were detected by spectrophotometry. The standard deviation of this method was ±0.032, and its minimum detectable concentration was 0.01μg·ml^(-1). The recovery rate of 17KS and 17OHCS estimation was from 99.0% to 101.0%, with coefficients of variation less than 6.23%.