宫颈病变组织中双链RNA依赖的蛋白激酶表达

Expression of Double-stranded RNA-dependent Protein Kinase in Cervical Lesion Tissues

目的:检测宫颈癌及癌前病变组织中双链RNA-依赖的蛋白激酶(PKR)的表达,探讨PKR在宫颈病变发生发展中的意义及可能的机制。方法:选择临床病历资料和病理资料完整的宫颈癌患者共37例、同期宫颈上皮内瘤样病变(CINⅠ～CINⅢ)患者56例为研究对象,因子宫肌瘤等良性病变切除子宫的正常宫颈组织30例作为对照。采用免疫组织化学SABC法检测PKR的表达,分析PKR在宫颈病变发生、发展中的作用。结果:宫颈癌组织中PKR表达阳性率为54.1%(20/37),宫颈癌分期等临床病理资料均无明显相关(P>0.05);宫颈癌组PKR表达高于CINⅢ,CINⅠ～Ⅱ组和正常宫颈组,差异有统计学意义(P<0.05)。结论:PKR的异常表达在宫颈病变的发展过程中可能具有重要作用。

Objective： To determine the expression of double-stranded RNA-dependent protein kinase （PKR） in cervical cancer and pre-malignant lesion tissues, and discuss its significance and possible mechanisms in the occurrence and development of cervical lesion. Methods： The cases with complete medical records and pathological information were registered in this study. 93 cases, including 37 cases of cervical cancer and 56 cases of cervical intraepithelial neoplasia （CIN Ⅰ - Ⅲ ）, were selected as research objects, meanwhile 30 cases experienced hysterectomy caused by benign lesion such as hysteromyoma were selected as controls. Immunohistochemical SABC assay was adopted to determine the expression of PKR. The roles of PKR in the occurrences and developments of cervical lesion were analyzed. Result： The positive rate of PKR expression in cervical cancer tissues was 54.1% （20/37）. There were no significant relationship between PKR expression and pathological data such as clinical staging （P〉0.05）. PKR expression in the cervical cancer group was significantly higher than the CIN Ⅲ, CIN Ⅰ - Ⅱ and normal cervical groups, with statistical differences （P〈0.05）. Conclusion： The abnormal expression of PKR may play important roles in the development of cervical lesions.