实验性全肠外营养状态下肠道免疫功能的保护

Study on gut immunity protection during total parenteral nutrition in rats

研究表皮生长因子（EGF）和谷氨酸胺（GLN）对全肠外营养（TPN）大鼠肠道免疫功能的保护作用．方法：应用大鼠TPN模型和高效液相色谱技术测定大鼠血浆及各组织GLN浓度；分离培养肠粘膜淋巴细胞测定IL-2活性；取肠系膜淋巴结做细菌培养．结果：常规TPN可导致血浆及各组织GLN明显下降，肠粘膜淋巴细胞IL-2活性明显下降，细菌易位增高；而在TPN过程中加用外源性GLN，可维持血浆及各组织的GLN浓度，防止肠粘膜淋巴细胞IL-2活性下降，减少细胞易位；EGF可防止肠道GLN水平下降，提高肠道对GLN的摄取率；并可有效防止肠粘膜淋巴细胞IL-2活性的下降；减少细菌易位．结论：EGF和GLN具有保护TPN状态下肠道免疫功能的作用．

Aim: To evaluate the effects of epidermal growthfactor (EGF) and glutamine (GLN) on gut immunity in ratswith total parenteral nutrition (TPN). Methods: The concentration of GLN was determined by high-performance liquid chromatography (HPLC). The lamina propria lymphocytes of intestine were isolated and cultured for measuringIL-2. Mesenteric lymph nodes were harvested for bacterialstudy. Results:TPN induced marked reduction of GLN concentration in tissues and blood. IL-2 production of gut lymphocyte decreased and the bacterial translocation (BT) increased. However, when EGF was administered along withTPN, gut GLN concentration did not fall down and gutGLN extraction was increased, the IL-2 production of gutlymphocyte increased and the BT decreased significantlycompared with those in TPN group. Conclusion: EGF andGLN can protect the gut immunity during TPN.