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鸡背根神经节及脊髓中钙通道α_2亚基的表达-背根神经节中其与calbindin-D28k共存研究

Immunohistochemical demonstration of the calcium channel α_2 subunit in the chicken dorsal root ganglion and spinal cord-A special reference to colocalization with calbindin-D28k in dorsal root ganglion neurons
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摘要 目的观察鸡脊髓及背根神经节(DRG)中钙离子通道α2(CCα2)亚基的免疫学表达,并观察在DRG中CCα2与calbindin-D28k(CB)的共表达的相互关系。方法通过免疫组织化学,观察了鸡背根神经节中表达情况,利用图像分析系统对VDCC-α2δ-1亚单位免疫阳性和阴性神经元数量和细胞面积进行测量,统计出不同大小细胞的构成比。结果在脊髓背角I、II层和背内侧束(LT)内,CCα2δ亚基免疫阳性呈点状样分布于神经终末之中。在DRG神经元中,约72%的神经细胞呈CCα2免疫阳性,其中小型至中型神经元占免疫阳性神经元的88%。约75%的CCα2免疫阳性神经元与CB共存,其中大部分(90%)神经元是小、中型神经元。结论CCα2主要表达于伤害性神经元中,并在DRG伤害性感觉神经元中,伤害性信息的传递可能与细胞的Ca2+调节机制,即通过CCα2δ亚基的Ca2+内流和细胞内CB的Ca2+结合活性这两种因素有关。 Objective To study the distribution of the calcium channel α2 (CCα2)subunit in the chicken spinal cord and dorsal root ganglion(DRG)neurons and determine its relationship with calbindin-D28k (CB) in the DRG neurons. Methods Used immunohistochemieal methods, the number and size of immunoreactive(IR) neurons were measured by using a image analyze system, and were compared with those of negative neurons. Results CCα2 subunit was detected in nerve terminals, which were observed as dot-like structures, and in laminae I, II,III and Lissauer' s tract in the spinal dorsal horn. In the DRG neurons,approximately 72% of the total neurons were CCα2 subunit positive,and most(88% )of these neurons were small to medium sized. A majority(75% )of the positive neurons showed CB immunoreaetivity and most(90% )of these neurons were small to medium sized. Conclusion It is postulated that the mode of nociceptive transmission may involve a cellular Ca^2+ -regulating system that consists of both Ca^2+ entry via CCs with the α2 subunit and intracellular Ca^2+-binding activity of CB in the nociceptive neurons of the DRG.
出处 《中国实用医药》 2009年第20期1-2,共2页 China Practical Medicine
关键词 钙通道α2亚基 CALBINDIN-D28K 脊髓 DRG 免疫组织化学 CCα2 subunit Calbindin-D28k Spinal cord DRG immunohistochemistry
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参考文献7

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