病毒性肝炎肝组织P16蛋白的表达及其意义

Study on expression of P16 protein in the liver tissue of viral hepatitis

目的了解病毒性肝炎肝组织中Ｐ１６的表达及其意义。方法对４８例病毒性肝炎、７例原发性肝癌病人肝组织进行了Ｐ１６免疫组化研究。结果Ｐ１６蛋白在慢性肝炎轻、中度的阳性率分别为８５．００％和９１．６６％，而在慢性肝炎重度的阳性率仅为５０．００％，肝硬变慢性重型肝炎、原发性肝癌中没有表达，慢性肝炎轻、中度的阳性率均明显高于肝硬变慢性重型肝炎、原发性肝癌的阳性率（Ｐ＜０．０１，０．００１），进一步证明肝硬变是原发性肝癌的癌前病变。与病原学关系表明，Ｐ１６在乙型肝炎中表达的阳性率为７７．１４％，明显高于在丙型肝炎中的表达（Ｐ＜０．００１）。结论丙型肝炎更易导致原发性肝癌的发生。

P16 protein is a cell cycle regulator involved in the genesis of many types of tumors. In order to study the signifi cance and expression of P16 protein in liver tissue of viral hepatitis,48 patients with viral hepatitis and 7 cases with hepatocarcinoma were investigated by immunohistochemistry.The results showed that the positive rate of P16 protein expressed in the liver tissues of mild (85.0%) and moderate (91.66%)chronic viral hepatitis were significently higher than that in liver cirrhosis and hepatocarcinoma (P<0.01,0.001). this suggess that the expression of P16 plays an important role in the carcinogenesis.