慢性乙肝病毒感染者HBV DNA前C区A83基因突变的研究

A study on HBV DNA pre C region A83 mutationin patients with hepatitis B

用错配聚合酶链反应（Ｍ－ＰＣＲ）及限制性片段长度多态性分析（ＲＦＬＰ）方法，对９７例慢性乙肝病毒感染（ＨＢＶ）者血中ＨＢＶＤＮＡ前Ｃ区Ａ８３基因突变进行检测。结果显示：共检出Ａ８３基因突变株感染３７例（３８．１％），其中重症肝炎和重度慢性肝炎患者的检出率均明显高于轻度慢性肝炎和ＨＢＶ携带者；ＡＬＴ明显升高者的检出率明显高于ＡＬＴ正常或升高不明显者；ＨＢｅＡｂ（＋）者明显高于ＨＢｅＡｇ（＋）者。

Mismatched polymerase chain reaction(MPCR) and restriction fragment length polymorphism assay(RFLP) were performed to detecte the HBV DNA pre core A83 mutation in 97 patients with chronic HBV infection in Hunan. HBV DNA pre core A83 mutation were detected in 37 out of 97 patients (37/97,38.1%). It was found that the prevalence of HBV DNA pre core A83 mutation of the patients with severe hepatitis and with CAH(chronic active hepatitis) were significantly higher than those with CPH(chronic persistent hepatitis) and carriers of HBV, and the patients with elevated transaminase (ALT>70U) or positive HBeAb were significantly higher than those with normal transaminase (ALT<70U) and with positive HBeAg, respectively. The results suggest that the HBV DNA pre core A83 mutation is associated with active and severe liver tissue in patients with hepatitis B.