摘要
用错配聚合酶链反应(M-PCR)及限制性片段长度多态性分析(RFLP)方法,对97例慢性乙肝病毒感染(HBV)者血中HBVDNA前C区A83基因突变进行检测。结果显示:共检出A83基因突变株感染37例(38.1%),其中重症肝炎和重度慢性肝炎患者的检出率均明显高于轻度慢性肝炎和HBV携带者;ALT明显升高者的检出率明显高于ALT正常或升高不明显者;HBeAb(+)者明显高于HBeAg(+)者。
Mismatched polymerase chain reaction(MPCR) and restriction fragment length polymorphism assay(RFLP) were performed to detecte the HBV DNA pre core A83 mutation in 97 patients with chronic HBV infection in Hunan. HBV DNA pre core A83 mutation were detected in 37 out of 97 patients (37/97,38.1%). It was found that the prevalence of HBV DNA pre core A83 mutation of the patients with severe hepatitis and with CAH(chronic active hepatitis) were significantly higher than those with CPH(chronic persistent hepatitis) and carriers of HBV, and the patients with elevated transaminase (ALT>70U) or positive HBeAb were significantly higher than those with normal transaminase (ALT<70U) and with positive HBeAg, respectively. The results suggest that the HBV DNA pre core A83 mutation is associated with active and severe liver tissue in patients with hepatitis B.
出处
《湖南医科大学学报》
CSCD
1998年第3期269-271,共3页
Bulletin of Hunan Medical University