用逆转录-套式聚合酶链反应检测我国不同临床型肝炎/肝病患者中庚型肝炎病毒感染

Detectin of hepatitis G virus infection among clinical patients with hepatitis/liver diseases in China by reverse transrciption-nested polymerase chain reaction

目的为了研究庚型肝炎病毒（ＨＧＶ）在我国的感染状况。方法根据已发表的ＨＧＶ的５’端非编码区（５’－ＵＴＲ区）及螺旋酶区（ＮＳ３区）两段高度保守的基因序列分别设计两套引物，用逆转录－套式聚合酶链式反应（ＲＴ－ｎｅｓｔｅｄＰＣＲ）检测ＨＧＶＲＮＡ。结果从北京、秦皇岛、河南等地采集各种肝病患者及职业献血员血清３５４份，ＨＧＶＲＮＡ阳性７９份，阳性率为２２．３％。其中已确定的临床型肝炎／肝病患者２５４例，ＨＧＶＲＮＡ阳性者为５０例，阳性率为１９．６％。原因不明的或非甲～戊型肝炎患者４３例，ＨＧＶＲＮＡ阳性者为１３例，阳性率为３０．２％。丙型肝炎阳性的职业献血员５７例，ＨＧＶＲＮＡ阳性者为１６例，阳性率为３０．２％。结论提示ＨＧＶ感染在我国多种人群中普遍存在，它不仅可能是引起非甲～戊型肝炎的重要病原之一。

According to two highly conserved genome sequences withinthe helicase(NS3)region and 5'-uncoding(5'UTR)region,we designed two sets of primer pairs to detect HGV RNA by RT-nested PCR in order to study HGV infection in Chinese population.Three hundred and fifty-four serum specimens of various liverdiseases were collected from Beijing,Qin Huangdao and Henan areas.Seventy-nine out of 354(22.3%)specimens were HGV RNA positive.Among 254 known clinical hepatitis/liver disease samples,50(19.6%)were HGV RNA positive.Thirteen HGV RNA positive samples(30.2%)were derived from 43 cryptogenic or nonA-E hepatitis. In 57commercial blood donors who were antibody positive to HCV 16(30.2%)were HGV RNA positive,suggesting HGV infection is common in various population.It may be an etiological factor which leads to nonA-E hepatitis and post-transfusion hepatitis.