庚型肝炎患者肝组织中HGV表达的免疫组化研究

Immunohistochemical study of HGV expression in liver of patients with hepatitis G

目的探讨庚型肝炎病毒（ＨＧＶ）在庚型肝炎肝组织中的表达状况与临床意义．方法应用免疫组织化学ＰＡＰ方法以鼠抗ＨＧＶＮＳ５单克隆抗体对庚型肝炎患者２０例（急性肝炎２例，慢性肝炎８例，肝硬变１０例，血清ＨＧＶＲＮＡ皆阳性）肝组织中ＨＧＶ抗原进行检测．结果庚型肝炎患者２０例中，８例（４０％）肝组织中检出ＨＧＶ抗原；不同病期检出率分别为：急性肝炎０／２（０％），慢性肝炎２／８（２５％），肝硬变６／１０（６０％），各组间差异无显著意义；阳性信号位于肝细胞胞质；阳性细胞可位于炎症坏死灶周围；抗原阳性与阴性组间肝组织炎症活动度及血清谷丙转氨酶水平无明显差别，但阳性组纤维化指数较高．

AIM To study the expression and its significance of hepatitis G virus (HGV) in liver tissues of patients by HGV infection. METHODS HGV antigen was detected in 20 hepatitis G patients (acute hepatitis 2, chronic hepatitis 8, cirrhosis 10, serum HGV RNA was positive for all patients) by immunohistochemical PAP method using monoclonal antibody to synthetic peptide encoded from HGV NS 5 gene. RESULTS Among 20 patients with hepatitis G, HGV antigen was detected in 8(40%). The detection rates in acute hepatitis, chronic hepatitis, and liver cirrhosis were 0/2 (0%), 2/8 (25%), and 6/10 (60%), respectively. No difference of the positive rate was found among the three group patients. The positive signal was located in the cytoplasm of hepatocytes, and the stained cells could be found located at or near necroinflammatory areas. The fibrosis index in HGV antigen positive patients was higher than in HGV antigen negative ones, while there was no obvious difference in the inflammatory activity index and the serum ALT level. CONCLUSION HGV infection and its expression in liver might be correlated with liver fibrosis.