白细胞介素Ⅰ对登革病毒感染人血管内皮细胞的影响

Effect of IL 1β on dengue virus infection of human vein endothelial cells

目的：探讨白细胞介素Ⅰ（ＩＬ１β）在登革病毒发病中的作用，以及登革病毒感染的发病机理。方法：用不同浓度的ＩＬ１β分别作用于登革Ⅱ型病毒（Ｄ２Ｖ）感染人脐静脉内皮细胞（ＨＵＶＥＣ）的不同环节（病毒感染前、感染同时、感染后）。于感染后不同时间收集感染上清液，用微量蚀斑法滴定病毒产量。结果：ＩＬ１β对人脐静脉内皮细胞中的Ｄ２Ｖ增殖有抑制作用。ＩＬ１β在实验浓度范围内不造成血管内皮细胞形态和数量的改变。结论：ＩＬ１β在防止或减轻登革病毒感染发病中起重要作用。

Objective:To study the effect of IL 1β on the pathogenesis of dengue fever.Methods:IL 1β in different concentrations were incubated separately with human unmbilical vein endothelial cells(HUVEC) before,during and after D 2V infection.Culture supermatants were collected and titrated for virus concentrations by microplaque forming assay at different time intervals from 24 to 96 hours postinfection.Results:IL 1β inhibited the multiplication of D 2V in EC.Within the range of IL 1β concentrations used in the experiment,no changes in morphology or number of cells were found both in normal or D 2V infected HUVEC.Conclusions: IL 1β plays an important role in auceliocating the dengue virus infection.