浙江省丽水山区134份犬脑组织狂犬病病毒抗原检测

Detection of rabies virus antigen in 134 dog brain samples in Lishui

目的对浙江省丽水市山区犬脑组织标本进行狂犬病病毒抗原检测,了解犬狂犬病毒感染状况。方法收集丽水市山区狂犬病和疑似狂犬病犬以及外观健康犬脑组织标本134份,用直接免疫荧光试验(DFA)检测狂犬病病毒抗原,确定阳性标本。结果134份犬脑组织标本印片DFA检测9份为阳性,占所检标本的6.72%(9/134),其中外观异常并攻击人/犬导致人或犬间疫情的犬脑组织标本8份,均阳性;外观正常犬脑组织标本共126份,1份阳性,阳性率0.79%(1/126)。结论在丽水市首次用免疫学方法进行狂犬病病毒的实验室检测,狂犬病病毒在丽水山区狂犬病疫区的犬间相互传播,犬狂犬病疫情存在扩散的现象。

Objective To investigate the infection status of rabies virus in dogs by detecting the virus antigen in dog brain samples in the mountain area in Lishui, Zhejiang province. Methods A total of 134 brain samples from the sick dogs, the suspected sick dogs and the dogs looking health were taken, and the virus antigen was detected by using direct fluorescent antibody method （DFA）. Results Nine samples were positive, accounting for 6.72% （9/134）, and eight were from the dogs which looked abnormal and bit people or other dogs causing human or dog rabies. Among 126 brain samples of the dogs looking health, one was positive with the positive rate of 0.79% （1/126）. Conclusion It was the first time to use DFA to detect rabies virus in Lishui, which indicated that rabies virus transmitted in dogs in the endemic area and the dog rabies epidemic was in expansion.