日本血吸虫不同发育阶段与旋毛虫抗原交叉性的研究

Identification of antigens shared between Schistosomajaponicum and Trichinella spiralis

运用ＥＩＴＢ技术分析日本血吸虫（Ｓｊ）尾蚴、肝期童虫及雌雄成虫与旋毛虫（Ｔｓ）肌蚴抗原的交叉性。结果显示：Ｓｊ尾蚴、肝期童虫、成虫各阶段不同程度地被抗－ＴｓＳＡＲＳ，ＴｓＩＲＳ识别，其识别的抗原分子量在１５～１００（尤５０～７０）ｋＤ之间，两种抗血清识别的带型基本相同。所识别的各期Ｓｊ抗原中以尾蚴抗原最多，次为肝期童虫。３０天成虫仅雌虫抗原与上述两种抗血清在９７ｋＤ处显出一条弱带。抗－ＳｊＡＲＳ，ＳｊＩＲＳ识别ＴｓＳＡ中抗原成份分子量基本在３４ｋＤ以上，以３４ｋＤ和３５～３８ｋＤ带最为明显。提示Ｔｓ与Ｓｊ之间存在较多的交叉抗原，主要在幼虫阶段。

Cross-reactivity was analysed between the antigen of T.spiralis muscular larvae and antigens of cercariae, liver stage schistosomula, 30 day male and female adult worms of S.japonicum by means of EITB technique. The result showed that the antigens of cercariae schistosomula, female worms, except male worms, were recognized by sera from rabbits immunized with T.spiralis antigens or by sera from T.spiralis infected rabbits. The molecular weight of recognized antigens ranges from 15 to 100kD, mainly from 50 to 70kD. The immunized rabbit sera and the infected rabbit sera reacted with the same cross-antigens of S.japonicum. Among these schistosome stages, the cercariae showed the most cross-reactive bands, the second were the liver stage schistosomula, and 30 day female worms only showed a weak band which located at 97kD position. Meanwhile, the anti-S.japonicum sera recognized several T.spiralis antigens which located at 34kD and above, especially 35～38kD.The results suggest that there are many antigens shared between T.spiralis and S.japonicum, and the main crossreactive antigens are found in the larva stages of S.japonicum and T.spiralis muscular larva. The crossreative antigens are different between various stages of S.japonicum and muscular larva of T.spiralis.