摘要
运用EITB技术分析日本血吸虫(Sj)尾蚴、肝期童虫及雌雄成虫与旋毛虫(Ts)肌蚴抗原的交叉性。结果显示:Sj尾蚴、肝期童虫、成虫各阶段不同程度地被抗-TsSARS,TsIRS识别,其识别的抗原分子量在15~100(尤50~70)kD之间,两种抗血清识别的带型基本相同。所识别的各期Sj抗原中以尾蚴抗原最多,次为肝期童虫。30天成虫仅雌虫抗原与上述两种抗血清在97kD处显出一条弱带。抗-SjARS,SjIRS识别TsSA中抗原成份分子量基本在34kD以上,以34kD和35~38kD带最为明显。提示Ts与Sj之间存在较多的交叉抗原,主要在幼虫阶段。
Cross-reactivity was analysed between the antigen of T.spiralis muscular larvae and antigens of cercariae, liver stage schistosomula, 30 day male and female adult worms of S.japonicum by means of EITB technique. The result showed that the antigens of cercariae schistosomula, female worms, except male worms, were recognized by sera from rabbits immunized with T.spiralis antigens or by sera from T.spiralis infected rabbits. The molecular weight of recognized antigens ranges from 15 to 100kD, mainly from 50 to 70kD. The immunized rabbit sera and the infected rabbit sera reacted with the same cross-antigens of S.japonicum. Among these schistosome stages, the cercariae showed the most cross-reactive bands, the second were the liver stage schistosomula, and 30 day female worms only showed a weak band which located at 97kD position. Meanwhile, the anti-S.japonicum sera recognized several T.spiralis antigens which located at 34kD and above, especially 35~38kD.The results suggest that there are many antigens shared between T.spiralis and S.japonicum, and the main crossreactive antigens are found in the larva stages of S.japonicum and T.spiralis muscular larva. The crossreative antigens are different between various stages of S.japonicum and muscular larva of T.spiralis.
出处
《湖南医科大学学报》
CSCD
1998年第3期225-228,共4页
Bulletin of Hunan Medical University
基金
湖南省科委资助
关键词
日本血吸虫
旋毛虫
抗原类
交叉反应
Trichinella spiralis
Schistosoma japonicum
enzymelinked immunoelectrotransfer blot
antigens,helminth
cross reaction