载脂蛋白B基因MspI多态性分析及其临床应用

Analysis of apolipoprotein B gene MspI,polymorphism and its clinical application

为研究载脂蛋白Ｂ（ａｐｏＢ）基因多态性与冠心病的关系，应用ＰＣＲ检测９０例冠心病患者和１２０例健康人ａｐｏＢＭｓｐＩ酶切位点的限制性片段长度多态性（ＲＦＬＰ）。结果产生三种基因型：Ｍ＋Ｍ＋、Ｍ＋Ｍ－、Ｍ－Ｍ－，冠心病组等位基因Ｍ＋、Ｍ－频率分别为０．９２、０．０８；对照组Ｍ＋、Ｍ－频率分别为０．９８、０．０２，少见等位基因Ｍ－的频率在冠心病组显著高于对照组（Ｐ＜０．０５），但两组各基因型之间的血脂、脂蛋白、载脂蛋白水平均无显著性差异。提示ａｐｏＢ基因该位点的多态性可能与冠心病的发病有关。

The purpose of this study was to analyze the relation between apolipoprotein B geen polymorphism and coronary heart disease(CHD).ApoB gene MspI RFLP was observed in 90 patients with CHD and 120 controls by PCR.Three genotypes were examined:M+M+、M+M-、M-M-.The distributions of apoB gene frequencies were 092 for allele M+、008 for rare allele M- in CHD group,and 098 for allele M+、002 for rare allele M- in controls.The gene frequency of rare allele M- was higher in CHD group than in controls(P<005).However,no significant differences in lipids、lipoproteins and apolipoproteins of every genotype were observed in patients and in controls.The gene polymorphism was related with CHD,but not with plasma lipids lipoproteins and apolipoproteins.[WT5”HZ〗