可降解海藻酸钠-壳聚糖支架用于细胞黏附生长的研究

Controlled-biodegradable alginate-chitosan scaffolds for cell adhesion

通过调节海藻酸钠与壳聚糖的比例制备了具有不同降解速度的组织工程支架,并以HepG2为细胞模型考察可降解支架对细胞黏附生长的影响。研究表明:壳聚糖含量越高,支架被溶菌酶降解的速度越快,但支架在培养液中的稳定性越高;MTT结果显示细胞在壳聚糖含量为100%和67%的支架中培养时活性较高,但活死染色显示细胞多以分散状态黏附在支架上,降低壳聚糖含量时细胞活性较低且多以聚集形式存在于支架空隙内部。通过调节海藻酸钠与壳聚糖的比例制备出可降解的组织工程支架,可以控制细胞的生长速度及黏附状态,有望为细胞的生长及功能发挥提供更适宜的环境。

Alginate-ehitosan scaffolds were prepared for their biodegradation and cell adhesion in tissue engineering. The increase of ehitosan in the scaffold resulted in faster biodegradation and enhanced mechanical property. Though nearly all seeded cells maintained viability according to MTT assay, small clusters were seen scattered within the sponge. Spheroid formation was better with increase of alginate, which may promote functional expression of cells. Our studies showed that controlling the ratio of alginate and ehitosan in the scaffold would provide a biodegradable and conducive environment to facilitate the performance of cultured cells by enhancing their attachment and aggregation.