应用RNA干扰技术对甜菜夜蛾信息素结合蛋白功能的研究

Functional study of pheromone binding protein by using RNAi in male beet armyworm,Spodoptera exigua Hübner

采用RNA干扰技术,通过腹腔注射法将甜菜夜蛾信息素结合蛋白1(SexigPBP1)双链RNA导入雄蛾体内,48 h后定量PCR检测表明,SexigPBP1的mRNA表达量被敲减90%以上;同时,触角电位(EAG)测定结果表明,注射处理48 h后雄蛾对雌蛾性信息素主组分Z9,E12-14∶Ac的EAG反应降低了约60%,证明SexigPBP1在雄蛾对该组分的感受中起重要作用。

Pheromone binding proteins （PBPs） are thought to play critical roles in male perception of conspecific female sex pher- omone. The dominant method to address the functions of PBP is in vitro binding assays between PBP and sex pheromone chemicals. The present paper dealt with the PBP function study by using RNA interference technique. At two days after injection of SexigPBP1 dsRNA into the moth abdomen cavity, the mRNA expression level of SexigPBP1 in treated moths, determined by quantitative real- time PCR, reduced by above 90% compared with the level in water-injected control moths. Meantime, the electroantennography （EAG） response to the major sex pheromone component Z9,E12-14 ： Ac reduced significantly by about 60% in RNAi treated moths with contrast to the control moths, which provides a direct evidence that SexigPBP1 plays important role in perception of Z9,E12,14 ： Ac in Spodoptera exigua.