杜仲内生真菌的分离鉴定及其抑菌活性研究

Isolation and Identification of Endophytic Fungi from Eucommia ulmoides and Study of Their Antimicrobial Activities

从药用植物杜仲中分离到41株内生真菌,综合运用形态特征和分子生物学技术,明确其分类地位,分别属于粗糙脉孢霉(Neurospora crassa)、曲霉属(Aspergillus)、小菌核属(Sclerotium)、盾壳霉属(Conio-thyrium)、离蠕孢属(Bipolaris)、白僵菌属(Beauveria)6个属,并进行抗植物病原真菌活性检测,结果表明48.8%的内生真菌对植物病原菌有生长抑制作用,除1株白僵菌属菌株外对G+、G-细菌均有明显的抑菌作用.

Forty-one strains of endophytie fungi were isolated from the branches of Eucommia ulmoides in Sichuan province. They were classified and identified to six genera according to their morphological and micro-structure characters, e.g. Neurospora crassa, As- pergillus, Sclerotium, Coniothyrium, Bipolaris, Beauveria. Antibacterial activities of 41 strains endophytic fungi were tested with phyto- pathogen fungi. The result shows that the percentage of antifungal strains is 48.8%. Most strains prominently have inhibitive actions to all tested phytophogen fungi and bacterium（ G ＋ , G- ） except Beauveria.