Pluronic嵌段共聚物磁性纳米颗粒的制备及其对漆酶的分离纯化

Preparation of Pluronic-based Magnetic Nanoparticles and Their Application in Purification of Laccase

选择合适的双亲性嵌段共聚物Pluronic产品接枝到Fe3O4粒子的表面,合成了粒径均一、亲水性好、非特异性吸附低的纳米级磁性分离载体(PMNPs).通过对漆酶发酵液的分离纯化研究了Pluronic对排除蛋白非特异性吸附的作用.结果表明,PMNPs对漆酶的最大吸附量可达0.535mg/mg,在5～40℃内基本不受温度变化影响,对漆酶发酵液的分离纯化因子为3.4,酶活收率达62.9%,可实现一步分离提纯.

Nano-sized monodispersed Pluronie （PEO-PPO-PEO）-based magnetic nanoparticles （PMNPs） were synthesized as carders for the purification of laccase from the mycelium culture of Trametes versicolor. The adsorption assay was carried out in model system oflaccase. The result showed that the maximum adsorption capacity was 0.535 mg/mg without any decrease in the range from 5℃ to 40 ℃. Purification of laccase from mycelium culture by PMNPs was also investigated. Total enzyme activity recovery rate could reach 62.9% with a single step, and purification fold 3.4.