压痕试验法研究递增负荷训练对大鼠松质骨生物力学性能的影响

Indentation Test to Study the Effect on Biomechanical Property of Spongy Bone in Rats Under Progressively Increasing Load Training

利用压痕试验法研究递增负荷训练对SD大鼠松质骨的生物力学性能的影响。100只健康3月龄雌性SD大鼠随机分为5个训练周期(8 W、9 W、11 W、13 W和15W)的运动组(8W-T,9W-T…15W-T)及相应的对照组(8W-C,9W-C…15W-C),每组各10只。运动组进行递增负荷大强度跑台训练。采用压痕法测定第一腰椎椎体松质骨的生物力学性能指标,并与平行实验中用压缩法得到的结果进行比较。结果发现:训练周期接近9 W时,运动组松质骨的最大载荷开始低于对照组,至11 W时,运动组与对照组间开始表现出显著性差异(P=0.0004),并一直持续至15 W;压缩法的测量结果也是在11 W表现出显著性差异,但两者并不完全一致。最后结论:压痕试验可以很好地检测出松质骨生物力学性能的变化;与压缩法相比,压痕试验法对松质骨力学性能进行测定更具优势。

Objective Indentation test is a useful method to study biomechanical property of bone. In this study,the spongy bone of SD rats under progressively increasing load train-ing was tested. Method 100 healthy SD rats of 3 months old were used as samples after adaptive exercise for one week,and they were divided to 5 control groups and 5 exercise groups according to 8W,9W,11W, 13W and 15W cycle of training. The rats in exercise groups were under progressively increasing load training. The biomeehanieal property of the 1st lumbar vertebrae （L1） was tested by indentation test. Results （1） To the 9W exercise group,the maximal elastic force （F） of spongy bone was less than control group, remarkable difference was found from llW which P=0. 0004,and it continued to 15W. （2） The result achieved by compression test also showed remarkable difference from 11W,but there were differences between the two methods. Conclusions （1） Indentation test is a valid method to measure the changes of spongy bone＇s properties. （2）Compared to compression test, indentation test makes the samples purely, and affected by less factors.