原发性肝癌与乙型肝炎病毒基本核心启动子和前C区基因变异的相关性

The association of primary liver cancer with the mutations in basic core promoter and precore genes of hepatitis B virus

目的 研究原发性肝癌（PLC）与HBV基本核心启动子（BCP）和前C区基因变异的相关性。方法对144例HBsAg（＋）PLC患者的血清进行HBV标志物和HBVDNA检测，对HBeAg（-）但HBVDNA（＋）的样本再采用实时荧光定量PCR进行前C区和BCP区基因变异检测。随机选取120例慢性乙型肝炎（CHB）患者作为对照组。结果PLC患者血清HBV标志物中HBeAg（＋）46例，占31．94％，HBeAg（-）98例，占68．06％。在98例HBeAg（-）患者的血清中，HBVDNA（＋）56例，占57．14％，其中前C1896变异43例，占76．79％，BCP1762／1764基因变异50例，占89．29％，共同变异38例，占67．86％。PLC患者中的前C1896和BCP1762／1764基因变异率高于CHB患者，且差异有统计学意义（X^2值分别为9．36和5．77，P值均〈0．05）。结论PLC的发生可能与前C区和BCP区基因变异有关。

Objective To investigate the association of primary liver cancer （PLC） with the mutations of HBV preeore and basic core promoter （BCP） genes. Methods The serum markers of hepatitis B and the quantities of serum HBV DNA were detected in 144 HBsAg-positive PLC patients. The precore and BCP gene mutations in patients with HBeAg-negtive and HBV DNA-positive were detected by real-time PCR. One hundred and twenty chronic hepatitis B （CHB） patients were randomly selected to serve as the control. Results There were 46 （31.94%） patients with HBeAg-positive and 98 （68.06%） patients with HBeAg-negative. In 98 HBeAg-negative patients, 56 （57. 14% ） were HBV DNA-positive, in which 43 （76.79%） were with precore 1896 gene mutations, 50 （89.29%） were with BCP1762/1764 gene mutations, and 38 （67.86%） were with both geue mutations. Precore 1896 and BCP1762/1764 gone mutation rates in PLC patients were much higher than those in CHB patients （X^2 = 9. 36 and 5.77, P 〈 0. 05）. Conclusion PLC may be associated with the mutations of HBV preeore and BCP genes.