内毒素对大鼠肝星状细胞NF—κB mRNA表达及醛固酮分泌的影响

Effects of endotoxin on NF-κB mRNA expression and aldosteron secretion in rat hepatic stellate cells

目的探讨内毒素对大鼠肝星状细胞（HSC）核转录因子κB（NF—κB）p65 mRNA表达及醛固酮分泌的影响。方法将培养后的HSC—T6分为内毒素组和对照组，内毒素组采用1．0μg／mL的内毒素进行处理。放射免疫法检测HSC分泌醛固酮的情况，一步法FIT—PCR检测HSC表达NF—κB p65 mRNA的水平。结果内毒素作用6、12、24和48h时，醛固酮分泌显著高于对照组（t值分别为3．063、4．577、6．847和9．317，P值均〈0．05），NF—κB p65 mRNA的表达与对照组比较，差异有统计学意义（t值分别为5．155、6．095、7．875和9．313，P值均〈0．01）。HSC中醛固酮的分泌与NF—κB p65 mRNA的表达呈正相关（r=0．889，P〈0．01）。结论内毒素可以上调HSC分泌醛固酮并使NF—κB p65 mRNA的表达上调，这种效果随着内毒素作用时间的延长而更为显著，可能是内毒素致肝纤维化形成的机制之一。

Objective To investigate the effects of endotoxin on nuclear factor-κB 1965 （ NF-κB p65 ） mRNA expression and aldosteron secretion in rat hepatic stellate cells （HSCs）. Methods Cultured rat HSCs （HSC-T6） were divided into endotoxin-treated group and control group. Cells in endotoxin-treated group were exposure to 1 mg/mL endotoxin. Aldosteron secretions of HSCs were determined by radioimmunoassay, and NF-κB p65 mRNA expressions of HSCs were detected by one-step RT-PCR. Results At 6, 12, 24 and 48 h, aldosteron secretions in endotoxin-treated group were significantly higher than those in the control group （ t = 3. 063,4. 577,6. 847 and 9. 317, P 〈 0. 05 ）, and the expressions of NF-κB p65 mRNA in endotoxin-treated group were also higher than those in control group （t =5. 155, 6. 095, 7. 875 and 9. 313, P 〈 0. 01 ）. Aldosteron secretions and NF-κB p65 mRNA expressions in HSCs displayed a positive correlation（ r = 0. 886,P 〈 0.01 ）. Conclusion Endotoxin can up-regulate the aldosteron secretion and NF-κB p65 mRNA expression in rat HSCs, which may be one of the mechanisms of liver fibrosis induced by endotoxin.