血管内皮生长因子对早产大鼠高氧肺损伤保护作用的研究

An experimental study on VEGF protection of hyperoxia-induced lung injury of premature rat

目的研究血管内皮生长因子(VEGF)在早产儿高氧肺损伤中的保护作用,寻找防治新生儿慢性肺疾病的新途径。方法建立高氧肺损伤动物模型,对早产大鼠随机分为3组:空气对照组、高氧组和高氧+VEGF组,高氧组和高氧+VEGF组的氧浓度控制在90%以上。分别于第4、14天处死动物,用实时PCR的方法检测肺组织VEGF和血小板内皮细胞黏附分子1(PECAM-1)mRNA的表达水平,HE染色对肺组织进行形态学观察。结果肺组织VEGF和PECAM-1 mRNA的表达水平在高氧组降低(P<0.05),而高氧+VEGF组与空气对照组比较差异无统计学意义(P>0.05)。高氧组在第4天可见肺间质增宽,成熟的肺泡数目减少,可见较多囊泡。第14天时上述变化更加明显,成熟的肺泡少见。通过加用VEGF干预后,上述病变减轻。结论VEGF对高氧肺损伤具有保护作用。

Objective To study the protection of VEGF in yperoxia-induced lung injury of premature rats. Methods A hyperoxia-induced lung injury animal models was first established. Premature rats were randomly assigned into three groups： a control group, high oxygen group and the high-oxygen ＋ VEGF group. The oxygen concentrations of high-oxygen group and the high oxygen ＋ VEGF group oxygen is were controlled more than 90%. On the 4th day, the animals were sacrificed, and use real-time PCR detection methods to detect PECAM-1mRNA and VEGFmRNA expression level,and use HE staining for conventional morphological observations. Results VEGF mRNA and PECAM-1 mRNA expression level was lower in the high-oxygen group, and high oxygen ＋ VEGF group compare with a control air group without differences. High-oxygen group in four days was a qualitative thicker interstitium lungs, the pulmonary alveolus reduction in the number of mature, and to see more blasttule. In paragraph 14 of the climate change was more evident mature pulmonary alveolus scarcely. Through the use of VEGF intervention, the pathological changes reduced. Conclusion VEGF has a hyperoxia- induced lung injury protection.