急性坏死性胰腺炎生长抑素治疗后血内毒素、TNF—α和肝TNF—αmRNA的变化

THE CHANGES OF SERUM ENDOTOXIN,TNF-α AND HEPATIC TNF-α MRNA IN RAT ACUTE NECROTIZING PANCREATITIS MODELS AFTER THE TREATMENT OF SOMATOSTATIM

目的探讨急性坏死性胰腺炎（ANP）的血内毒素，TNF—α和肝TNF—αmRNA表达的变化以进一步探讨多器官功能障碍综合征（MODS）的发生机制，以及生长抑素的治疗作用。材料和方法将SD大鼠随机分为ANP组、ANP十生长抑素组和正常对照组。经胰胆管道行注射3．5％牛磺胆酸建立大鼠ANP模型后测定血内毒素和TNF—α的浓度以及肝TNF—αmRNA表达。结果ANP大鼠血内毒素、TNF—α和肝TNF—αmRNA表达增强，但在生长抑素治疗后均见降低。结论TNF—α在ANP的肝损伤中起重要作用，生长抑素可以减轻肝损伤，对TNF治疗有效。

Objectives By evaluating the changes of serum endotoxing, TNF-α and liver TNF-αmRNA expression in rat acute necrotizing pancreatitis (ANP),the mechanism of multple organ dysfunction syndrome (MODS) has been further investigated.Methods SD rats were randomly divided into ANP,ANP with somatostatin therapy and normal control groups. After biliopancreatic duct injection of 3. 5 % sodium taurocholate to establish the ANP model,Serum endotoxin,TNF-α concentrations and theexpression of liver TNF-αmRNA were measured. Results Serum endotoxin concentration and liver TNF-αmRNA expression increased in ANP rats,but all of them decreased after somatostatin therapy. Conclusions TNF-α playes an important role in injuring liver in cases of ANP,somatostatin could alleviate the severity of liver damage,so it carried the beneficial effect in treatment ofANP.