诱变处理苜蓿愈伤组织抗碱性的研究　　

Study on alkalinity resistance of alfalfa callus in mutagenic treatments

以苜蓿Medicago sativa茎段为试验材料,对其进行愈伤组织的诱导,采用紫外线和NaN3化学诱变处理苜蓿愈伤组织。对诱变处理和对照的愈伤组织先进行脯氨酸筛选,再进行NaHCO3和Na2CO3碱性胁迫,并进行过氧化物酶(POD)、可溶性糖和脯氨酸含量等抗碱性生理指标鉴定。结果表明:诱变处理3种生理指标趋于一致,均比未经处理的高。紫外线辐射和NaN3化学诱变的方法均可用于提高苜蓿的抗碱性。其中紫外线诱变要比NaN3化学诱变更有利于提高苜蓿的抗碱性。

The stem of alfalfa（Medicago sativa）was used as the experimental material, the inducement of callus was conducted, and the callus of alfafa was treated by ultraviolet ray and NAN3. Callus in mutagenic treat and control were filtrated by proline and forced alkalinity stress by NaHCO3 and Na2CO3, then they were appraised by the alkalinity resistance physiological indexes such as peroxidase activity, soluble protein content and proline content. It was shown that all these three indexes in the treated callus were evidently higher than those in the control. So the method of ultraviolet ray and NaN3 could be used to improve the alkalinity resistance of alfalfa, and ultraviolet ray treatment had greater effect on the alkali resistance of alfalfa than NaN3 treatment.