铁皮石斛试管苗培养技术的研究

Study on Vitro Culture Techniques of Dendrobium candidum

研究了不同量激素浓度、天然添加物及植入密度对石斛试管苗生长的影响。结果表明：使用1/2MS培养基并且在其他条件不变的情况下,当6-BA的含量在3.0 mg/L时,丛生芽数量多且有原球茎的发生 香蕉汁具有明显的壮苗和诱导芽增殖的效果 当铁皮石斛茎段植入密度为10~12个/瓶时,对其分蘖和生长有利。

Dendrobium candidum is one of the rare Chinese herbal medicines which are in danger. In order to further understanding the artificial propagation system, the experiment studied the effects of different hormone levels, nature addictives and implantation densities. The results Showed that in the same circumstance, using 1/2 MS culture medium, when 6-BA in the content of 3.0 mg/L, there was a larger number of buds and also PLBs occur, banana juice had an obvious effect on sprouts strength and bud-induced proliferation, it is good for the tiller and the growth of Dendrobium candidum, when the implantation density was 10-12/bottle.