摘要
采用MS基本培养基,添加不同种类和不同浓度的植物激素,选择无污染的北五味子种子,进行组织培养随机试验,筛选北五味子诱导分化较适合的培养基。结果表明:E(MS+NAA0.3 mg/L+2,4-D 0.5 mg/L+GA30.6 mg/L+6-BA 2.0 mg/L+蔗糖30 g/L+琼脂8 g/L)处理对北五味子诱导成活率最高,对北五味子种子诱导效果最好。
Adopting MS basic culture medium, adding different kinds and different concentration of the plant hormones, choosing non-pollution seeds of Schisandra sphenanthera Rehd. et Wils, cloing the random testing of tissue culture. Result Shoued The E treatment had the best sunival rate of Schisandra sphenanthera Rehd. et Wils's induction, also had the best effect on the seeds of Schisandra sphenanthera Rehd. et Wils.
出处
《北方园艺》
CAS
北大核心
2009年第7期242-244,共3页
Northern Horticulture
基金
吉林省教育厅科研资助项目
关键词
北五味子
组织培养
培养基
Schisandra sphenanthera Rehd. et Wils
Tissue culture
Culture medium