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复方鹿仙草颗粒含量测定方法研究 被引量:1

Determination of the Content of Matrine and Oxymatrine in Fufang Luxiancao Granule
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摘要 目的:建立测定复方鹿仙草颗粒中苦参碱和氧化苦参碱含量的HPLC方法,用以控制复方鹿仙草颗粒的质量。方法:色谱柱为氨基柱;流动相为乙腈-无水乙醇-3%磷酸(81∶9.5∶9.5);检测波长为220nm;流速1.0mL/min.结果:苦参碱在0.0676-2.028μg范围内,氧化苦参碱0.1533-4.599μg范围内线性关系良好(r苦参碱=0.99994,r氧化苦参碱=0.99999),平均加样回收率苦参碱为102.5%,氧化苦参碱为97.4%(RSD苦参碱=2.1%,RSD氧化苦参=0.97%,n=6)。结论:本法灵敏、快速、准确,可用于复方鹿仙草颗粒的质量控制。 Objective: To establish a HPLC method for the determination of the content of matrine and oxymatrine in Fufang Luxiancao Granule.Methods: The chromatographic column was NH2 column;mobile phrase was acetonitrile-ethyl alcohol-3% phosphoric acid(81:9.5:9.5) with a detection wavelength of 220nm at the flow rate of1.0 ml/min.Results: The matrine and oxymatrine showed a good liner relation at the range of 0.0676-2.028μg and at the range of 0.0676-2.028μg respectively(matrine r=0.99999) and 0.1533-4.599μg(oxymatrine r=0.99999).The average recovery rates of the matrine and oxymatrine were 102.5%(RSD=2.1%) and 97.4%(RSD=0.97%) respectively.Conclusion: The HPLC method is sensitive,rapid and accurate and can be used to control the quality of Fufang Luxiancao Granule.
出处 《云南中医中药杂志》 2009年第7期55-56,共2页 Yunnan Journal of Traditional Chinese Medicine and Materia Medica
关键词 复方鹿仙草颗粒 含量测定 苦参碱 氧化苦参碱HPLC Fufang Luxiancao Granule content determination matrine oxymatrine HPLC
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