摘要
本文介绍了利用超高效液桐色谱串联质谱（UPLC／MS/MS）快速检测鸡肉中吡虫啉残留量的方法。样品通过乙睛-水（体积比为9：1）溶液提取，饱和乙睛的正己烷净化。采用电喷雾电离，正离子模式，多反应监测扫描。该方法的最低检出限为0．1μg／kg，在0．1—100μg／kg的线性范围内，相关系数r为0．9978，三种不同加标浓度，7次平行实验平均回收率为87．47％～98．85％，相对标准偏差（RSD）为3．09％～5．29％，该方法具有前处理简单，灵敏度高，分析时间短等优点，适用于鸡肉中吡虫啉农药残留的快速检测。
A method for detemlination of imidacloprid residue in chicken by Ultra Performance Liquid Chromatography-Tandem Mass Speelrometry （UPLC/MS/MS）has been developed. The samples were extracted by mixed acetonitrile and water mixed solution （9：I,V/V）, and cleaned up with hexane saturated with acelonilrile. Identification was achieved by electrospray ionization in positive mode ESI＋ using mulliple reaction monitoring （MRM）. The limit of detection was 0.1 μ g/kg, The linear ranges were from 0.1 to 1 00 μg/kg with correlation coefficients of 0.9978. The average recoveries were in the range of 87.47% - 98.85% with three different spiked levels, and the relative standard deviations ranged from 3.09% to 5.29% （n=7）. The method can be applied for the fast determination of imidaeloprid residue in chicken because of its sample preparation, higher sensilivity and shorter analysis lime.
出处
《福建分析测试》
CAS
2009年第3期10-13,共4页
Fujian Analysis & Testing
