摘要
本文建立超高效液相色谱一串联质谱(UPLC—MS/MS)快速测定水产品中三聚氰胺残留的方法。色谱条件:ACQUITYUPLCBEHHILIC柱(2.1×100mill,1.7m),流动相:乙腈-0.5mmol/L酸铵溶液(0.1%甲酸),流速:0.3mL/min。采用电喷雾质谱检测,以正离子模式5min就完成了质谱分析。实验结果表明,三聚氰胺在水产品中的检测限为0.05mg/kg,在0.05—0.50mg/kg添加水平时加标回收率为63%-90%之间,相对标准偏差(RSD,n=6)均小于7.2%。
The residue of melamine in aquatic product was studied by ultra performance liquid chromatography-tandem mass spectrometer. The sample was extracted and separated on ACQUITY UPLC BEH HILIC column (2.1 × 100 mm, 1.Tin) with acelonitrile-0.5 mmol./L ammonium acetate (0.1% formic acid) as the mobile phase at flow rate 0.3 mL/min. The detection was performed by tandem mass spectrometry, with electrospray ionization in positive mode (ES+) in five minutes. Experimental results shoowed that the limit of detection was 0.05 mg/kg, the recoveries between 0.05-0.50 mg/kg levels ranged from 63% to 90%. Relative standard deviations (n=6) were less than 7.2% .
出处
《福建分析测试》
CAS
2009年第3期23-26,共4页
Fujian Analysis & Testing
关键词
三聚氰胺
水产品
超高效液相色谱-串联质谱
melamine
aquatic products
ultra performance liquid chromatography-tandem mass spectrometer