PPAR-γ配体对巨噬细胞源泡沫细胞形成及TLR-2、TLR-4表达的影响

Effects of PPAR-γ Ligand on the Mice Peritoneal Macrophage Foam Cells Formation and TLR-2,TLR-4 Expression Induced by OxLDL

目的观察PPAR-γ配体GW1929对小鼠腹腔巨噬细胞源泡沫细胞的形成及TLR-2、TLR-4表达的影响。方法oxLDL(50μg/mL),GW1929(20μmol/L)作用于小鼠腹腔巨噬细胞24h,油红O染色观察各组泡沫细胞的形成。采用激光共聚焦显微镜观察各组TLR-2和TLR-4的表达。结果对照组及GW1929组细胞几乎未被油红O染色,oxLDL组大量细胞胞质被油红O染成暗红色,而oxLDL+GW1929组细胞胞质染色明显浅于oxLDL组细胞。oxLDL组泡沫细胞TLR-2和TLR-4的表达明显高于control与GW1929组,而oxLDL+GW1929组泡沫细胞TLR-2和TLR-4的表达明显低于oxLDL组(P<0.05)。结论GW1929可能通过抑制oxLDL诱导巨噬细胞表面TLR-2、TLR-4的表达上调,抑制泡沫细胞的形成,从而起到抗动脉粥样硬化的作用。

Objective To observe the effects of PPAR-γ ligand, GW1929 on the mice peritoneal macrophage foam cells formation and TLR-2, TLR-4 expression induced by oxLDL. Methods The mice peritoneal macrophages were pretreated with oxLDL （ 50μg/mL） and GW1929 （20μmol/L） respectively for 24 h. Then they were stained by oil red O to observe foam cells formation. Then confocal fluorescence microscopy was used to observe effects of GW1929 on foam cells TLR-2 and TLR-4 expressions. Results The mice peritoneal macrophages of control group and GW1929 group are not almost stained by oil red O, but the macrophages of oxLDL group become dark red after stained by oil red O, and the cells color of oxLDL＋GW1929 group is thinner than that of oxLDL group. Expressions of TLR-2, TLR-4 in oxLDL group are higher than those in control and GW1929 group obviously, but expressions in oxLDL＋GW1929 group are lower than oxLDL group respectively. Conclusion PPAR-γ ligand, GW1929 probably stable atheromatous plaque through inhibiting foam cells foamation and expressions of TLR-2, TLR-4.