婴幼儿皮肤血管瘤组织中半胱氨酸天冬氨酸蛋白酶-3和内皮抑素的表达

Association of caspase-3 and endostatin expression in infantile dermal hemangiomas

目的检测婴幼儿皮肤血管瘤不同病理时期血管瘤组织中半胱氨酸天冬氨酸蛋白酶-3 (caspase-3)和内皮抑素的表达,了解血管瘤的生物学特性,探讨血管瘤的增生及消退机制。方法采用免疫组化链霉菌亲生物素蛋白过氧化物酶连接法检测43例手术切除的婴幼儿皮肤血管瘤标本及6例正常皮肤组织标本中caspase-3和内皮抑素的表达情况,同时用末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷三磷酸生物素缺口末端标记法测定同一标本的细胞凋亡情况。结果消退期血管瘤中caspase-3和内皮抑素阳性表达以及凋亡指数均高于增生期(P<0.05),增生期二者的阳性表达均高于正常皮肤组织表达(P<0.05)。caspase-3与内皮抑素的阳性表达呈正相关(r=0.753,P<0.01)。结论caspase-3和内皮抑素可能通过促进和诱导内皮细胞的凋亡使血管瘤由增生期进入消退期,内皮抑素可增加caspase-3的活性,caspase-3和内皮抑素在血管瘤的发生发展中发挥重要协同作用,有利于血管瘤的自然消退。

Objective To investigate the expression and significance of caspase-3 and endostatin in different stages of infantile dermal hemangioma, and to understand the difference between two stages of hemangioma and normal skin. The athogenesis of hemangioma was studied. Methods The immunohistochemical streptavidin-peroxidase method was used to detect the expression caspase-3 and endostatin in proliferating and involuting hemangioma and normal skin tissues. The apoptotic ceils in tissues of hemangioma were detected by terminal deoxynucleotide transferase- mediated deoxyuridine triphosphate-biotin nick end-labeling. Results The positive expression of caspase-3 and endostatin and apoptosis index was significantly higher in involuting phase than that in proliferating one （P 〈0.05）. There was also significant difference between proliferating hemangioma and normal skin tissue （P 〈0.05）. Thus the expression of Caspase-3 and endostatin were positively associated. Conclusion Caspase-3 and endostatin may play important roles in apoptosis of endothelial cells to transfer the course of proliferative phase to involuting phase. Endostatin can up-regulate the activity of capase-3 and have synergistic action in hemangiomas pathological development which may accelerate its regression.