视觉与听觉通路诱导的豚鼠眨眼条件反射的比较研究

Comparison research of the classical eyeblink conditioning in guinea pig induced by visual and auditory circle

目的以眼肌电记录法分别记录豚鼠以声音和光信号作为条件刺激（CS）的眨眼条件反射（CR），分析并比较其习得规律。方法采用金属电极埋植技术及简易“随动”气流给气刺激装置，分别以声音和光信号作为条件刺激（CS），以气流作为非条件刺激（US），以眼肌电信号判断其眨眼行为，在清醒无制动的豚鼠上进行经典眨眼条件反射的训练。结果在豚鼠内眦采集并记录到眨跟肌电信号显示，声音条件刺激组在第1天、第4天、第7天、第10天的CR习得率分别为（5．0±1．09）％、（31．0±2．09）％、（61．334-1．63）％、（82．33±1．63）％，而光条件刺激组在第1天、第4天、第7天、第10天的CR习得率分别为（5．5±4．59）％、（4．33±3．14）％、（10．83±13．42）％、（3．83±1．33）％，2组CR习得率差异有显著性（P〈0．05）。结论以声音作为条件刺激时豚鼠能够成功建立的眨眼条件反射，而以光作为条件刺激时眨眼条件反射的建立却非常困难。

Objective To record the classical eyeblink conditioning single, which the conditioned stimulus（CS） is tone or light, by the eleetromyographie （EMG） of museuli oeuli recording. Methods Adopting metal eleetrode embedding teehnie and simple air puff stimulus facility,tone or light as the eonditioned stimulus（CS） was paired with air puff being uneonditioned stimulus（US） to establish elassieal eyeblink conditioning of conscious unfixed guinea pigs. The eyeblink behavior were evaluated through EMG of museuli oeuli. Results The eyeblink EMG signal of museuli oculi showed ： when the tone as the CS , the results indicated that the acquisition rate of the 1 s,, the 4th, the 7th, the 10th day were respectively （ 5.0 ± 1.09 ） %, （ 31.0 ± 2.09 ） %, （ 61.33 ± 1.63 ） %, （ 82.33 ± 1.63）% ;when the light as the CS ,the results indicate that the acquisition rate of the 1st ,the 4th ,the 7th ,the 10thdaywererespeetively （5.5 ±4.59）%,（4.33±3.14）%,（10.83±13.42）%,（3.83±1.33）%. Conclusion While CS is tone,classical eyeblink conditioning can be successfully established for the guinea pigs;on another hand,while CS is light,it is very hard for the guinea pigs to establish the classical eyeblink conditioning.