抗肺癌合剂对Lewis肺癌的抑制作用及VEGF表达的影响

The Depressant Effect on Lewis Lung Carcinoma and the Effect on the Expression of VEGF by Melting Agent of Anti-lung Cancer(MAALC)

[目的]观察抗肺癌合剂对C57BL/6小鼠Lewis肺癌的抑制作用,并从分子水平观察其对肿瘤组织VEGF表达的影响。[方法]C57BL/6近交系小鼠50只,造模后,抗肺癌合剂(低、中、高3个剂量)、生理盐水、CTX分别作用于动物模型。15d后处死所有动物,剥取瘤组织称重,并计算抑瘤率;应用免疫组化法及图像分析技术检测瘤组织VEGF表达的积分光密度。[结果]在平均瘤重上,中药高剂量组与模型组有显著性差异(P<0.05);在肿瘤组织VEGF表达上,中药高剂量组与模型组有显著性差异(P<0.05)。[结论]抗肺癌合剂能抑制Lewis肺癌小鼠肿瘤生长,并能降低Lewis肺癌小鼠肿瘤组织VEGF表达。

[Objective]To study the effect on the growth of mouse Lewis lung carcinoma and on the expression of VEGF in tumor tissue.[Methods] C57BL/6 mice were inoculated the Lewis lung carcinoma cell at right armpit and divided into groups. Different groups were administered by MAALC （low, normal and high dose）, CTX or saline. After 15 days, all mice were put to death to detach the tumors then weighed them. After that, we treated tumor tissue with VEGF immunohistochemistry stain, detected the integral optical density of VEGF expression with image analytical technique. [Results]Compared with model group, the average weight and VEGF expression level of the tumors of the high dose group were less and statistically significant （P%0.05）. [Conclusion]MAALC can restrain the growth of Lewis lung carcinoma,and can degrade the expression level of VEGF in tumor tissue.