TPPA和RPR联合检测梅毒抗体的实验研究

TPPA and RPR Joint Detection of the Experimental Study of Syphilis Antibody

[目的]分别用TPPA和RPR两种方法检测梅毒抗体,比较两种方法在梅毒诊断及疗效观察方面的意义。[方法]对疑为梅毒患者血清标本共80份分别进行TPPA和RPR检测。[结果]63份梅毒患者TPPA阳性,阳性率78.75%,灵敏度100%;RPR39例阳性,阳性率51.25%,灵敏度61.90%。[结论]TPPA的灵敏度和特异性均优于RPR,故TPPA法检测梅毒特异性抗体,应作为临床梅毒确诊的主要检测手段,RPR法检测梅毒非特异性抗体,应作为临床梅毒的筛差实验以及梅毒治疗的监测手段。

[Objective] Compare the significance on syphilis diagnosis and curative effect by detection of syphilis antibodies using two methods, TPPA and RPR, respectively. [Methods] Using TPPA and RPR ways to detect 80 serum samples of suspected syphilis patients. [Results]63 samples were positive in syphilis test using TPPA. Positive rate was 78.75%, sensitivity 100%;39 samples were positive in syphilis test using TPPA. Positive rate was 51.25%, sensitivity 61.90%. [Conclusions]The TPPA is superior than RPR in sensitivity and specificity. Therefore, detecting specific syphilis antibody by TPPA can be used as the main method in clinical diagnosis of syphilis, while detecting non-specific syphilis antibody by RPP can be used as the screening test of syphilis patients and the monitoring method to cure syphilis.