上皮钙黏蛋白和连环蛋白120在鼻咽癌组织中的表达及相关性研究

Expression of E-cadherin and P^(120ctn) in nasopharyngeal carcinoma and their relationship

目的:研究鼻咽癌组织中上皮钙黏蛋白(E-caol)和连环蛋白120(P120ctn)的表达情况,探讨它们的相关性及与临床病理特征的关系。方法:应用免疫组织化学二步法,检测经4%甲醛固定,石蜡包埋的56例鼻咽癌标本和15例正常鼻咽黏膜上皮标本中E-cad、P120ctn的表达情况。结果:56例鼻咽癌组织中E-cad、P120ctn异常表达率分别为64.29%、67.86%,主要表现为细胞膜表达减弱,细胞膜表达减弱伴细胞胞质表达;15例对照组正常鼻咽黏膜上皮E-cad、P120ctn异常表达率均为6.67%,均差异有统计学意义(P<0.01)。鼻咽癌组织中E-cad、P120ctn在低分化癌组的异常表达率(71.43%、85.71%)明显高于高中分化癌组(42.86%,36.29%);在有颈部淋巴结转移组的异常表达率(80.00%、85.00%)明显高于无颈部淋巴结转移组(52.78%、58.33%);在Ⅲ～Ⅳ期鼻咽癌组的异常表达率(76.92%、84.62%)明显高于Ⅰ～Ⅱ期鼻咽癌组(46.66%、53.33%);均差异有统计学意义(P<0.05)。E-cad与P120ctn在56例鼻咽癌组织中共同表达和共同异常表达分别为12例和30例,有显著一致性,呈正相关(r=0.5217,P<0.01)。结论:E-cad与P120ctn的异常表达与鼻咽癌的分化程度、临床分期、颈部淋巴结转移密切相关。它们共同参与鼻咽癌发生、发展、浸润及转移过程。

Objective： To study the expression of E--cadherin and p^120ctn in nasopharyngeal carcinoma tissues, and to investigate their relationship and the relation with clinico-pathological features. Method：Two-step immunohistochemical staining was applied to detect the expression of E cadherin and p^120ctn in formalin fixation and paraffin-embedded specimens from 56 cases with nasopharyngeal carcinoma and 15 cases with normal nasopharyngeal epithelia. Result：The abnormal expression rates of E-cadherin and p^120ctn in the 56 cases of NPC tissues were 64. 29Y00 and 67.86Y0 respectively, mainly with reduction of expression membrane and with the expression of cytoplasm; 6.67% of the 15 comparative normal cases of nasopharyngitis had abnormal expression of E-cadherin and p^120ctn The differences were statistically significant. The abnormal expression rates of E-cadherin and p^120ctn in NPC tissues were 71.43% and 85. 71% respectively in low differentiated cancer group, which was obviously higher than the rates 42.86% and 36.29% in high and middle differentiated cancer group. The 80.00% and 85.00% abnormal expression rate in the group with cervical lymph node metastases was higher than that in the group without cervical lymph node metastases（52. 78%,58. 33%）. The abnormal expression rate of E-eadherin and p^120ctn（ 76. 92% ,84.62%） in the third and forth phases was higher than that in the first and second phases （46.66% ,53. 33%）. The differences were statistically significant （P〈0.05）. There were all together 12 co-expression cases of p^120ctn and E-cadherin and 28 abnormal co expression cases in the 56 cases of NPC tissues, which was of obvious consistency and correlation, with the relevant indexes： rs=0. 5217 and P〈0. 01. Conclusion： The abnormal expression of E-cadherin and p^120ctn is closely related to the degree of differentiation, clinical stage and cervical lymph node metastasis, and they join in the process of NPC initiation, progression, invasion and metastasis.